Extended Data Figure 6: (2R,6R)-HNK rapidly increases the frequency and amplitude of AMPAR spontaneous excitatory postsynaptic currents in the hippocampus.
From: NMDAR inhibition-independent antidepressant actions of ketamine metabolites
a, Representative traces of spontaneous excitatory postsynaptic currents (sEPSCs) mediated via AMPARs during baseline (before) and 20 min after drug administration. b, Example CA1 stratum radiatum interneuron recorded from a rat hippocampal slice. c–j, Twenty-minute exposure of (2R,6R)-HNK (e, i), but not (R,S)-(KET) (c, g) or (2S,6S)-HNK (d, h), increased AMPA sEPSCs frequency and amplitude compared to baseline. Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001 (see Supplementary Table 1 for statistical analyses and n numbers). SLM, stratum lacunosum-moleculare; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum.
