Extended Data Figure 8: Effects of (2R,6R)-hydroxynorketamine on protein and protein phosphorylation levels in synaptoneurosomes.
From: NMDAR inhibition-independent antidepressant actions of ketamine metabolites
a, b, A single administration of (R,S)-KET (10 mg kg−1) or (2R,6R)- HNK (10 mg kg−1), did not alter levels of mTOR or phosphorylated mTOR in the hippocampus 1 h (a) or 24 h (b) after injection. c–j, Administration of (R,S)-KET or (2R,6R)-HNK did not alter levels of mTOR/phosphorylated mTOR (c, d), eEF2/phosphorylated eEF2 (e, f), proBDNF/mBDNF (g, h), or GluA1/GluA2 (i, j), in the prefrontal cortex of mice. The values for the phosphorylated forms of proteins were normalized to phosphorylation-independent levels of the same protein. Phosphorylation-independent levels of proteins were normalized to GAPDH. Data are mean ± s.e.m, and were normalized to the saline-treated control group for each protein. Images are cropped; see Supplementary Fig. 1 for complete blot images. *P < 0.05 (see Supplementary Table 1 for statistical analyses and n numbers).
