Extended Data Figure 7: TRAP after cancer cell astrocyte co-culture.
From: Carcinoma–astrocyte gap junctions promote brain metastasis by cGAMP transfer
a, Schematic illustration of TRAP experimental set up to isolate translating mRNA from MDA231-BrM2 cells under three conditions (1, 2 and 3). b, Principle component (PC) analysis of TRAP mRNA sequencing. c, Scatter plot of log2 fold changes regulated by astrocytes and gap junction communications between BrM cells and astrocytes. d, STAT1 and NF-κB p65 phosphorylation in H2030-BrM3 cells after a 2 h incubation with conditioned media from astrocyte co-cultures. Conditioned media samples were collected after 24 h co-culture of astrocytes with control or Cx43-depleted H2030-BrM3 cells (n = 3 independent experiments).
