Extended Data Figure 4: WNV infection of human hippocampal CA2/CA3 neurons with loss of synapses within the hippocampal CA1 and the entorhinal cortex.
From: A complement–microglial axis drives synapse loss during virus-induced memory impairment
a, Immunostaining of human WNV encephalitis and control post-mortem hippocampal tissue for WNV-antigen. Shown at high magnification are neuron cell bodies (arrows) and neurites (arrowheads) within the hippocampal CA2/CA3 region. b, c, Immunostaining within the hippocampal CA1 (b) or entorhinal cortex (c) for the presynaptic marker, synaptophysin, within human WNV encephalitis and control autopsy cases. Quantification of the per cent of synaptophysin+ area (hippocampal CA1 P = 0.3, entorhinal cortex P = 0.11 by two-tailed Student’s t-test (not significant). Scale bar, 20 μm. In one WNV encephalitis patient sample, the entorhinal cortex could not be quantified because it was missing from the section.
