Extended Data Figure 7: Expression of lineage-specific genes and mitochondrial OXPHOS genes in control and ePNT embryos.
From: Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease
a, Heatmap showing log2-transformed RPKM values of selected differentially expressed genes in trophectoderm (n = 10), epiblast (n = 10) and primitive endoderm (n = 10) lineages. b, Heatmap showing expression of mitochondrial OXPHOS genes after unsupervised hierarchical clustering. Expression of OXPHOS genes encoded by mtDNA is variable both within and between blastocysts. Control and ePNT samples cluster together, irrespective of whether the cytoplast and karyoplast had the same (blue font) or different (purple font) mtDNA. Sample numbers and blastocyst grades are shown. The reference population includes a previously published series14. Autologous and homologous ePNT samples are derived from blastocysts in which the karyoplast and cytoplast had the same mitochondrial genome. Heterologous ePNT samples were derived from pairs of zygotes with different mitochondrial genomes (Extended Data Fig. 5b). Expression levels are indicated on a high-to-low scale (purple–white–green). Source data files are available online for a and b.
