Extended Data Figure 10: Heteroplasmy in subclones of the hES cell line derived from 36PNT.
From: Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease
a, The 36PNT hES cell line was frozen at passage 3 (after derivation), thawed and subcloned to monitor heteroplasmy arising from the karyoplast donor mtDNA. Six colonies (15–20) were randomly selected at the first post-thaw passage (P3) and clumps of cells were plated on 3 tissue culture wells; 5/6 colonies gave rise to 3 subclones, which were grown to P11. Subclones are distinguished by colour in the graphs. Each data point represents the mean of two technical replicates for a single cell sample. Source data file is available online.
