Extended Data Figure 1: Gene cluster of lugdunin and generation of S. lugdunensis IVK28-Xyl.
From: Human commensals producing a novel antibiotic impair pathogen colonization
a, The lugdunin genes are located on a 30-kbp operon. lugA–D encode the four NRPS, which are preceded by the putative regulator gene lugR. Encoded upstream are putative ABC transporter genes (lugE–H) and genes for proteins with no described function (lugI and lugJ). A type-II thioesterase, that functions as a repair enzyme for stalled PCP domains, is encoded between lugC and lugD. The 4’-phosphopantetheinyl transferase LugZ converts inactive PCPs (apo-PCP) into the active holo-form by attachment of the 4’-phosphopantetheine cofactor. Encoded downstream is a putative monooxygenase (lugM). The transposon insertion site of Tn917, generating the lugdunin-deficient mutant IVK28 M1, is indicated by a red arrow. b, The xylose-inducible lugdunin producer strain S. lugdunensis IVK28-xyl was generated by replacement of the regulator gene lugR by the xylAB promoter along with the xylR gene encoding a xylose-sensitive repressor. The erythromycin resistance cassette ermB was integrated for selection purposes.
