Extended Data Figure 6: Activation of SFONos1 neurons during eating does not require angiotensin signalling.
From: Thirst neurons anticipate the homeostatic consequences of eating and drinking
a, Experimental design to test whether angiotensin signalling is necessary for prandial thirst. b, Angiotensin blockers (INH) did not inhibit eating-induced activation of SFONos1 neurons or prandial drinking (n = 3 mice). c, Angiotensin blockers did not affect food consumption (n.s., two-tailed Student’s t-test, n = 3 mice). d, e, ARCAgRP neurons that control hunger are not reciprocally modulated by eating and drinking. d, Schematic of fibre photometry set up for recording the activity of ARCAgRP neurons. Scale bar, 100 μm. e, ARCAgRP neurons were rapidly inhibited when fasted mice were presented with chow, as previously reported27, but were unaffected when dehydrated mice were presented with water (n = 5 mice). Values are mean ± s.e.m. (error bars or shaded area).
