Extended Data Figure 6: Role of Tets and histone demethylases in EMT induction.
From: Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition
a, Expression levels of Tet1, Tet2 and Tet3 in Fh1fl/fl cells from RNA-seq data. b–d, Expression levels of Tet2 and Tet3 (b), miR-200 (c), and Cdh1 (E-cadherin) (d) in Fh1fl/fl cells upon combined silencing of Tet2 and Tet3. The results are presented as RQ with maximum values obtained from technical replicates. Actb and Snord61 were used as endogenous controls for mRNA and miRNA, respectively. e, Expression levels of the indicated miRNAs upon inhibition of histone demethylases by GSK-J4. Snord61 and Snord95 were used as endogenous controls. f, Expression of the indicated miRNAs in Fh1−/− cells incubated for 24 h with 5 mM DM-aKG measured by qPCR. Results were obtained from four (vehicle), five (Fh1−/−CL19 DM-aKG) or three (Fh1−/−CL1 DM-aKG) independent cultures and presented as RQ with maximum values, normalized to Snord95 as endogenous control. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.
