Extended Data Figure 8: MMF triggers EMT in FH-proficient cells.
From: Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition
a, Bright-field images of cells treated for 6 weeks with MMF. Arrows indicate the typical protrusion of cells of mesenchymal phenotype. Scale bar, 400 μm. b, OCR of the indicated cell lines treated chronically with MMF (as in Fig. 3). See Methods for drug concentrations. OCR was normalized to total protein content. Results were obtained from six (for mouse cells) or eight (for human cells) wells, data are shown ± s.d. c, Hive plot of metabolomics data of mouse and human cells treated with MMF (as in Fig. 3). All identified metabolites are included on the y-axis and grouped into human (pink) and mouse (green) cells. Metabolites accumulated (right x-axis) or depleted (left x-axis) in MMF-treated cells versus controls are indicated by a connecting arc and their fold-change is colour-coded. Metabolites accumulated commonly across the two cell lines are highlighted with a solid line. 2SC, 2-succinic-cysteine; succGSH, succinic-GSH. Source data are presented in Supplementary Table 2. Source metabolomic data are presented in Supplementary Table 3.
