Extended Data Figure 9: Succinate triggers EMT in Sdhb-deficient cells.
From: Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition
a, Intracellular succinate levels after incubation with 4 mM MMS measured by LC–MS. Data are presented as average ± s.d. b, c, Intracellular succinate (b) and succGSH (c) levels in Sdhb-deficient cells measured by LC–MS. Data are presented as average ± s.d. d, Bright-field images of cells of the indicated genotype. Scale bar, 400 μm. e, GSEA and EMT enrichment score from expression analysis of the indicated cell lines. f, g, miRNA expression levels normalized to Snord61 and Snord95 as endogenous controls (f) and CpG43 methylation (g). Experiments were performed as in Fig. 2b and d, respectively. Metabolomics experiments were obtained from five independent cultures. qPCR results were generated from three independent cultures. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Gel source images are presented in Supplementary Fig. 1. Source data are presented in Supplementary Table 2.
