Extended Data Figure 7: The transition from ncH3K4me3 to canonical H3K4me3 requires zygotic transcription but not DNA replication.
From: Allelic reprogramming of the histone modification H3K4me3 in early mammalian development
a, Box plots of gene expression levels (log2[FPKM + 1]) show the effects of α-amanitin and aphidicolin on genes activated during ZGA (left). Genes expressed at all stages (middle) and genes repressed at all stages (right) are also shown. b, Hierarchical clustering of two alleles of early embryos including those treated with α-amanitin or aphidicolin based on the global H3K4me3 enrichment. c, A snapshot of the UCSC browser view shows the global view of H3K4me3 enrichment for maternal and paternal alleles in various types of embryos including those treated with α-amanitin or aphidicolin. d, Bar charts showing normalized H3K4me3 and BrUTP fluorescence intensity of individual surrounded nucleolus (SN)-stage oocytes. Each pair of bars for H3K4me3 and BrUTP represents an individual oocyte. Data from three independent experiments were pooled. GV, germinal vesicle.
