Extended Data Figure 4: Reprogramming of H3K4me3 on the paternal genome.
From: Allelic reprogramming of the histone modification H3K4me3 in early mammalian development
a, A snapshot of UCSC genome browser shows the enrichment of H3K4me3 in sperm from a published data set10 and this study. b, Snapshots showing the allelic H3K4me3 signals and RNA-seq signals near the imprinted loci Snrpn and Impact. Please note the maternal RNAs in zygotes, early two-cell and late two-cell embryos are presumably inherited from oocytes. The paternal zygotic transcripts appear from the late two-cell stage onward for both genes. c, Bar chart showing the numbers of H3K4me3 peaks identified in sperm (in regions covered by SNPs) and the paternal alleles of early embryos. d, Hierarchical clustering of sperm, early embryos and somatic cortex based on the paternal allele H3K4me3 enrichment. For sperm H3K4me3 data, only regions covered by SNPs were included for analysis. e, A bar chart showing the percentages of raw reads assigned to the maternal or paternal allele for each stage. f, Scatter plots showing the H3K4me3 enrichment levels between SNP-assigned parental alleles from zygotes and pronuclei for both the paternal (left) and maternal genomes (right).
