Extended Data Figure 5: Non-canonical H3K4me3 (ncH3K4me3) in oocytes and early embryos
From: Allelic reprogramming of the histone modification H3K4me3 in early mammalian development
a, A snapshot of the UCSC browser view shows the enrichment of H3K4me3 in MII oocytes, isolated maternal pronuclei, PN5 zygotes (maternal allele), and histone H3 (maternal allele) in PN5 zygotes. b, Scatter plots showing the comparisons between the enrichment of histone H3 (maternal) in PN5 zygotes and H3K4me3 from either MII oocytes (left) or the maternal allele of PN5 zygotes (right). c, Hierarchical clustering of early embryo stages (maternal) based on global H3K4me3 enrichment. d, A bar chart showing the expression levels (log2[FPKM + 1]) of several marker genes for granulosa cells and oocytes, as well as a house-keeping gene (Actb), in oocyte samples collected at different stages. ICMs are included as a non-oocyte control. e, Heat maps showing the expression (FPKM) of all genes and the H3K4me3 enrichment (normalized RPKM) at their promoters (TSS ± 2.5 kb) for oocytes and early embryos. f, Hierarchical clustering of stages of oocytes and early embryos based on global H3K4me3 enrichment. g, A snapshot of UCSC browser shows the H3K4me3 enrichment in oocyte of various stages as well as DNA methylation in MII oocyte. A PMD with ncH3K4me3 enrichment is shaded.
