Extended Data Figure 3: Altered biology of Atg12cKO HSCs and characterization of Park2−/− mice.
From: Autophagy maintains the metabolism and function of young and old stem cells
a, Representative FACS plot of MTG staining in control and cKO HSCs. b, Representative electron micrographs depicting expanded small vesicles (top) and endoplasmic reticulum/Golgi (bottom) in control and cKO HSCs. Scale bar, 1 μm. c, Representative FACS plot and quantification of endoplasmic reticulum mass measured by ER-Tracker flow cytometry staining in control and cKO HSCs. d, e, Representative immunofluorescence images and quantification of (d) LAMP1 and (e) KDEL staining in control and cKO HSCs. Scale bars, 10 μm. f, Levels of p62 in control and cKO HSCs. g, Mitochondria parameters in Park2+/+ and Park2−/− HSCs. h–m, Characterization of Park2−/− mice: (h) scheme for analyses, (i) complete blood count parameters (left) and lineage distribution in peripheral blood (right), (j) bone marrow total cell numbers, (k) bone marrow mature populations, and (l, m) bone marrow immature populations. n, Transplantation of Park2−/− HSCs showing donor chimaerism (left) and lineage distribution (centre) in peripheral blood, and HSC chimaerism (right) at the indicated time after transplantation in primary and secondary recipients. Data are mean ± s.d. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
