Extended Data Figure 6: PI3Kδ blockade increases AID expression in human B-cell lines.
From: Phosphatidylinositol 3-kinase δ blockade increases genomic instability in B cells
a, Viable cells were counted at the indicated time points by Trypan Blue exclusion in MEC1, Mino, JeKo-1 and GM06990 cell lines treated with the indicated inhibitors (1 μM). b, Histograms showing the AICDA mRNA relative expression in JeKo-1 and GM06990 cell lines treated with 1 μM inhibitors. c, Western blot for AID protein expression in MEC1 and Mino B-cell lines treated with 0.1 μM inhibitors (n = 2 biological replicates). d, Western blot for AID protein expression in JeKo-1 and GM06990 B-cell lines treated with 1 μM inhibitors (n = 4 biological replicates). For gel source data, see Supplementary Fig. 1. e, Histograms showing the AID protein relative expression at the indicated time points in MEC1, Mino, JeKo-1 and GM06990 cell lines treated with 1 μM inhibitors. AID abundance was measured by ImageJ software and normalized for the β-actin intensity of the corresponding lane. Data are expressed as mean ± s.d. (n = 3 biological replicates). *P < 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, two-tailed Student’s t-test (a, b, e).
