Extended Data Figure 7: Linear unmixing on MARS solutions and MARS-dye-stained cells.

a, Three-channel epr-SRS images at 2,159 cm−1, 2,152 cm−1 and 2,145 cm−1 for 100 μM MARS2145, 1,000 μM MARS2152 and 300 μM MARS2159 before unmixing. b, Images after linear unmixing, with average readings of 94 μM, 1,097 μM and 315 μM for MARS2145, MARS2152 and MARS2159, respectively. c, Raw epr-SRS images for a 3-colour cell mix after each was stained with 1 μM MARS2237, 4 μM MARS2228 and 1 μM MARS2209 separately before linear unmixing. d, Images and their composite after linear unmixing.