Extended Data Figure 7: Molecular modelling of sCT peptide reveals potential interactions between peptide and receptor.
From: Phase-plate cryo-EM structure of a class B GPCR–G-protein complex
Cryo-EM density is shown in yellow fill, the sCT peptide model in yellow cartoon and the CTR in blue cartoon. a, Gln14 in sCT is predicted to form interactions with the backbone of ECL2. b, Ser5 and Thr6 are predicted to form hydrogen bonds with His302 in TM5 of the CTR, while Leu4 points down into the bundle towards TM6. c, Mutation of H302 to Ala (H302A) results in reduced potency for sCT in cAMP production (left) and phosphorylation of ERK1/2 (right) when expressed in 3T3-FlpIn cells. This supports a role H302 in sCT affinity. Data are mean + s.e.m. of four independent experiments performed in duplicate
