Extended Data Figure 3: Compiled data from transplantation experiments performed so far.

a, b, Total engrafted mice assessed in primary transplantation at various time points. a, Histogram of total mice injected with haemogenic endothelium cells and haemogenic endothelium cells infected with indicated library or constituent transcription factors. Haemogenic endothelium only: 2 out of 30 mice engrafted, none of which were multi-lineage (myeloid cells, erythroid cells, B cells and T cells); library: 11 out of 40 mice engrafted, 5 multi-lineage; 7 transcription factors: 33 out of 76 mice engrafted, 9 multi-lineage; 5TFpoly: 15 out of 30 mice engrafted, 5 multi-lineage. Engraftment was assessed from bone marrow at 4–16 weeks. b, Tables 1 and 2 show compiled data. c, Representative photomicrographs and FACS plots of robust EHT (top) and failed EHT (bottom). The appearance of round cells budding from adherent haemogenic endothelium cells by direct microscopic visualization was assessed routinely. RUNX1c+24 reporter positivity of haemogenic endothelium cells at day 3 of culture likewise reflected robustness of EHT (20% versus 5%).