Extended Data Figure 6: Profiling endogenous GSDME expression in the NCI-60 and effects of decitabine in GSDME-silenced cells.
From: Chemotherapy drugs induce pyroptosis through caspase-3 cleavage of a gasdermin
a, Lysates of 57 cancer cells from the NCI-60 (a panel of 60 diverse human cancer cell lines used by the US National Cancer Institute to screen and evaluate anti-cancer drugs) were subjected to anti-GSDME and anti-tubulin immunoblotting. Wild-type and GSDME−/− SH-SY5Y cells were included as the positive (PC) and negative control (NC), respectively. b, Six indicated GSDME-negative cancer cells were treated with 5 μM decitabine for 6 days and then stimulated with doxorubicin or actinomycin-D for cell death analyses. Top, qRT–PCR measurements of GSDME expression relative to GAPDH before and after decitabine treatment. Middle and bottom, LDH release-based cell death expressed as mean ± s.d. from three technical replicates. All data shown are representative of two independent experiments.
