Extended Data Figure 8: Endogenous GSDME in mouse EMT6 cells induces pyroptotic cell death in response to chemotherapy drug treatment.
From: Chemotherapy drugs induce pyroptosis through caspase-3 cleavage of a gasdermin
a, Immunoblotting profiling of GSDME expression in a panel of mouse cancer cells. b–e, EMT6 cells were treated with actinomycin-D and examined for cell death. b, Cell death determined by measuring LDH release (mean ± s.d.) from three technical replicates. c, Phase-contrast images of cell death morphology. d, Drug-induced cleavage of GSDME and effect of zVAD shown in anti-GSDME immunoblots. e, Effect of zVAD on cell death. A rabbit monoclonal antibody capable of recognizing the N-terminal domains of both human and mouse GSDME was used in a, d. All data shown are representative of three independent experiments.
