Extended Data Figure 10: GSDME mediates chemotherapy drug-induced tissue damage and toxicity in mice.

a–c, Wild-type or Gsdme^−/− mice were intraperitoneally injected with cisplatin or saline control. a, Representative haematoxylin and eosin staining of the small intestine (scale bar, 200 μm). White and black arrowheads indicate inflammatory cell infiltration and disappearance of the crypts, respectively. b, P values for the spleen damage data in Fig. 5c. c, Flow-cytometry profiling of the spleen cell population in one representative wild-type and one representative Gsdme^−/− mouse. CD3+ and B220+ mark the T and B cell lineages, respectively. d–g, Effects of Gsdme knockout on 5-FU-induced small intestine damage and bleomycin-induced lung damage. 5-FU was injected intraperitoneally at a dose of 250 mg per kg per day for 5 days (d, e) and 250 μg bleomycin (per mouse) was delivered intratracheally directly into the lung for 3 days (f, g). d, Left, representative images of the intestine. Right, representative haematoxylin and eosin staining of the small intestine (scale bar, 200 μm). f, Representative haematoxylin and eosin staining of lung sections (scale bar, 20 μm). Statistics for surviving crypts and villi in the small intestine (e) and quantification of lung inflammation and acute lung injury (g) are from 30 randomly selected fields from 3 mice (10 each) and expressed as mean ± s.d. Two-tailed unpaired Student’s t-test was performed in b, e, g (*P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant). All data shown are representative of two independent experiments.