Extended Data Figure 2: Sequence alignment of GSDME from human, mouse, zebrafish and lancelet.
From: Chemotherapy drugs induce pyroptosis through caspase-3 cleavage of a gasdermin
The alignment was generated using the ClustalW2 algorithm and presented using ESPript 3.0 (http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi). Identical residues are indicated by the dark red background and conserved residues are in red text. All GSDMEs share a gasdermin-N domain at the N terminus and a gasdermin-C domain at the C terminus, linked by a hinge loop. Zebrafish has two GSDMEs, GSDME1 and GSDME2 (previously known as DFNA5a and DFNA5b). The black box marks the caspase-3 cleavage motif in human and mouse GSDME and zebrafish GSDME1. No evident caspase-3-recognition motif could be found in the inter-domain linker regions of zebrafish GSDME2 or lancelet GSDME.
