Extended Data Figure 2: Metabolic tracing of [13C]l-methionine in Pten-prostate specific knockout mice.
From: mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer
a, Plasma LC/MS analysis of the indicated metabolite concentration after intravenous injection of [U-13C5]l-methionine (100 mg kg−1) in C57BL/6 mice at 3 months of age (time 0 min, n = 7 mice; time 10 min/60 min, n = 6 mice). The unlabelled (M + 0, 12C) and major labelled (13C, M + 4 or M + 5) metabolite concentration is presented in the histogram. Error bars, s.e.m. b, Experimental design of the [U-13C5]l-methionine (100 mg kg−1) in vivo. U-13C5-Met, l-methionine labelled with13C in five carbons;1 h, prostate samples extracted after 1 h pulse with [U-13C5]l-methionine; 10 h, prostate samples extracted after 10 h pulse with [U-13C5]l-methionine; c, Summary schematic of the alterations observed in the metabolomic analysis in Ptenpc−/− versus Ptenpc+/+. Spm, spermine; spd, spermidine; ODC1, ornithine decarboxylase 1; SpdS, spermidine synthase; SpmS, spermine synthase. d, Fractional labelling of the indicated metabolites from Fig. 1c. Median ± interquartile range (1 h (top), n = 4; 10 h (bottom), n = 3). FC, fold change. One-tailed Mann–Whitney U-test (d) was used for data analysis.
