Extended Data Figure 5: Aggregates are not cleared by macroautophagy.
From: A lysosomal switch triggers proteostasis renewal in the immortal C. elegans germ lineage
a, GFP::LGG-1-expressing germlines. b, Number of autophagosomes (mean ± s.d.) in the most proximal oocyte. c, Schematic of macroautophagy. d, GFP::LGG-1 after control or lgg-1 RNAi. e,f, Quantification of macroautophagy gene expression by RT-PCR. Normalized expression (mean ± s.d.) was scored for three biological replicates. ****P < 0.0001. The gel source data are shown in Supplementary Figure 1. g, h, GFP::RHO-1-expressing hermaphrodites treated with macroautophagy-gene RNAi. Percentage of animals with oocyte protein aggregation. Mean ± s.d. from three biological replicates, each of n = 50 animals. ****P < 0.0001. i, Matings after macroautophagy-gene RNAi. j, Time-lapse images of aggregate clearance. Bars, 5 μm.
