Extended Data Figure 7: Sperm-induced changes in mitochondrial morphology and ROS levels require V-ATPase function.
From: A lysosomal switch triggers proteostasis renewal in the immortal C. elegans germ lineage
a, MitoLS::GFP in germ cells of hermaphrodites. b, Different z-planes for MitoLS::GFP in the same distal germline. c, Proximal:distal MitoLS::GFP fluorescence ratios (mean ± s.d.) for n = 10 germlines. d, e, Proximal oocytes from MitoLS::GFP-expressing or MitoTracker CM-H2TMRos-stained females. f, g, Mitochondria from proximal oocytes in MitoLS::GFP-expressing females before and after mating. Mitochondrial lengths (mean ± s.d.). ****P < 0.0001. h, i, Proximal oocytes from MitoLS::GFP-expressing or MitoTracker CM-H2TMRos-stained hermaphrodites after vha-13 RNAi. Bars, 10 μm.
