Extended Data Figure 7: Dnmt3a-deficient CD8 T cells undergo effector differentiation.
From: Effector CD8 T cells dedifferentiate into long-lived memory cells
a, Summary of gp33-specific CD8 T cell quantities at effector and memory time points in lymphoid and nonlymphoid tissues. b, c, Summaries of viral titres in spleen (b) and day 5 lung and liver (c) of acutely infected wild-type and Dnmt3a cKO mice. d, Quantitative PCR analysis of Dnmt3a exon 19 recombination using a primer set that binds to DNA internal to the floxed target region. The mean and s.d. of intact (non-recombined) floxed Dnmt3a alleles were determined by quantitative PCR from four individually sorted gp33-specific effector and memory CD8 T cell populations. e, Real-time PCR analysis of Sell mRNA expression of naive and tetramer+ wild-type and Dnmt3a cKO effector CD8 T cells. f, Representative FACS analysis of Klrg1, CD127, CD27, and L-selectin expression on wild-type and Dnmt3a cKO effector and memory gp33-specific CD8 T cell splenocytes. g, Summary graph for the percentage of wild-type and Dnmt3a cKO L-selectin-positive gp276 and np396-specific CD8 T cells.
