Extended Data Figure 5: Ezh1 deficiency enhances embryonic HSPC engraftment.
From: Regulation of embryonic haematopoietic multipotency by EZH1
a, Whole E10.5 AGM and yolk sac were transplanted intravenously into sublethally irradiated NSG adult females. Chimaerism was monitored by retroorbital bleeding every 4 weeks. Representative flow plots are shown for analysis after 4 weeks in n ≥ 3 mice. See also Fig. 4e, f. b, Whole E9.5 PSP was transplanted intravenously into sublethally irradiated NSG adult females. Chimaerism was monitored via retroorbital bleeding every 4 weeks. Representative flow plots are shown for analysis after 8 weeks in n ≥ 3 mice. See also Fig. 4g. c, Representative flow plots of lineage analysis in E10.5 AGM Ezh1+/− and Ezh1−/− primary transplant recipients after 24 weeks, and in E9.5 PSP Ezh1+/− primary transplant recipient after 16 weeks (n ≥ 3 mice per group). See also Fig. 4e, g. d, Primary recipients in a were euthanized 24 weeks post-transplantation and 4 × 106 whole bone marrow was transplanted into sublethally irradiated adult NSG females intravenously. Chimaerism was monitored via retroorbital bleeding. Representative flow plots of E10.5 AGM and yolk sac secondary transplants after 4 weeks in n ≥ 3 mice. See also Fig. 4h. e, Left, secondary transplantation of E10.5 yolk sac primary recipients (Fig. 4f). Right, lineage distribution of E10.5 secondary recipients. Data are pooled across three independent experiments. *P < 0.05, ** P < 0.01 by unpaired two-sided t-test; see Supplementary Information for exact P values per time point.
