Extended Data Figure 8: Kymographs that show the constriction of FtsZ^55–56sGFP, FtsW–sGFP and MurJ–sGFP rings during cell division.

The strains ColFtsZ^55–56sGFP, ColFtsW-sGFP and ColMurJ-sGFP were imaged every 10 min in the absence (control) or presence (+PC) of PC190723 for a total of 60 min. MurJ–sGFP control kymographs were performed on cells in which the MurJ–sGFP signal appeared on the second frame to ensure that the entire constriction process was observed (that is, to confirm that the absence of a biphasic behaviour was not the result of only imaging cells in the later stages of cell division). FtsZ^55–56sGFP and FtsW–sGFP rings showed biphasic constriction behaviour (no or slow constriction, followed by fast constriction). The addition of PC190723 inhibited the constriction of larger FtsZ^55–56sGFP and FtsW–sGFP rings (top kymographs) but not of smaller rings that were undergoing fast constriction. MurJ–sGFP rings displayed only fast constriction, and therefore were always able to constrict in the presence of PC190723. Data are representative of three biological replicates. Scale bars, 0.5 μm.