Extended Data Figure 1: Switching fluorescent tags has no effect on protein co-localization data.
From: Peptidoglycan synthesis drives an FtsZ-treadmilling-independent step of cytokinesis
a, b, COL strains that express FtsZ–mCherry and FtsW–sGFP (a) or FtsZ–mCherry and MurJ–sGFP (b) were compared to strains that express FtsZ–CFP and FtsW–mCherry, and FtsZ–CFP and MurJ–mCherry, respectively (described in Fig. 2b, c). Scale bars, 2 μm. c, PCC values between fluorescence channels for each protein fusion pair were calculated for cells that showed septal FtsZ localization. From left to right, n = 138, 136, 133 and 139 cells. Negative PCC values are represented as 0. Data are represented as box-and-whisker plots in which boxes correspond to the first-to-third quartiles, lines inside the boxes indicate the median, and the ends of whiskers and outliers follow a Tukey representation. Statistical analysis was performed using a two-sided Mann–Whitney U test; ns, not significant. Images in a and b are representative of three biological replicates.
