Extended Data Figure 2: Antisense RNA fragments that target the DivIB–DivIC–FtsL complex increased cell volume and decreased protein expression.
From: Peptidoglycan synthesis drives an FtsZ-treadmilling-independent step of cytokinesis
a, Western blot that shows total protein extracts of ColJZpAS-DivIB after 1 h of antisense induction, and control ColJZpEPSA probed with antibodies against either PBP2A (loading control; upper bands) or DivIB (lower bands). b, Western blot that shows total protein extracts of ColJZpAS-DivIC after 1 h of antisense induction, and control ColJZpEPSA probed with antibodies against either PBP2A (loading control; upper bands) or DivIC (lower bands). Images in a and b are representative of three biological replicates. For gel source data, see Supplementary Fig. 1. c, Cell volume of cells that expressed antisense RNA against ftsL, divIB or divIC, or carried vector pEPSA (left to right, n = 421, 379, 279 and 361 cells). Data represented in column graphs in which the height of the column represents the mean and the whiskers are the s.d. Statistical analysis was performed using a two-sided unpaired t-test. ***P < 0.001; first versus second column, P = 3.50 × 10−6; first versus third column, P = 3.80 × 10−8; first versus fourth column, P = 1.27 × 10−29.
