Extended Data Figure 6: Locomotor behaviour of wild-type and genetically manipulated flies under ramping temperature cycles and under constant conditions after entrainment.
From: Circadian clock neurons constantly monitor environmental temperature to set sleep timing
a, Summary of heating index under ramping temperature cycles and free-running locomotor activity rhythms under constant conditions after entrainment to 18 °C to 25 °C ramping temperature cycles. per01, aristae-less nocte+ flies (nocte+ AL), aristae-less nocte1 mutants (nocte1AL) and Clk4.1m>UAS-TNT flies, in which synaptic transmission is blocked in DN1ps, displayed strong behavioural phenotypes during the heating phase of the temperature cycle as revealed by low correlation coefficients between activity and temperature during the heating phase (see Methods). per01, nocte1 mutants and flies lacking aristae displayed arrhythmic locomotor activity under constant conditions after entrainment to ramping temperature cycles. Data were presented as population average ± s.e.m. b, A summary of cooling indices (see Methods) for experimental and control flies. n values are shown in a. Data were presented as average of cooling index ± s.e.m. A one-way ANOVA was conducted and Tukey’s honest significant difference tests were used; *P < 0.05, **P < 0.005. n = number of flies. Individual P values are reported in the Source Data for this figure.
