Extended Data Figure 8: Activity and sleep profiles of chordotonal organ mutants and flies lacking aristae.

a, b, Locomotor activity averaged over four days of a ramping temperature cycle (left) and averaged sleep profiles (right) for the following flies: a, nocte⁺ control flies (blue, n = 46) and nocte⁺ flies that lack aristae (nocte⁺ AL, red, n = 28); b, nocte¹ mutants (blue, n = 56), and nocte¹ mutants that lack aristae (nocte¹ AL, red, n = 44). c, d, Locomotor activity profiles averaged over four days of a 22 °C to 28 °C (c) and a 18 °C to 28 °C (d) ramping temperature cycle for nocte⁺ flies (blue) and nocte¹nocte¹ mutants that lack aristae (nocte¹ AL, red) (left); averaged sleep profiles (right). The number of flies in c and d are shown in e. e, Summary of locomotor activity rhythms under the two different temperature cycling conditions for the genotypes indicated. Data were presented as population average ± s.e.m. f, Locomotor activity averaged over four days of a ramping temperature cycle are shown on the left and averaged sleep profiles are shown on the right. The genotypes tested were F-gal4/+ (blue), UAS-nocteRNAi1/+ (green) and F-gal4> UAS-nocteRNAi1-AL (red), where AL refers to the aristae-less condition. The number of flies is shown in h. g, Anticipation of the onset of cooling in the genotypes indicated. F-gal4>UAS-nocteRNAi1-AL flies are largely arrhythmic (AR), so the anticipation index was not calculated (see Methods). Data are population average ± s.e.m. n values are shown in panel h. h, Summary of locomotor activity rhythms under constant conditions after entrainment. Data are mean ± s.e.m. i, Locomotor activity averaged over four days of a 18 °C to 25 °C ramping temperature cycle (left); averaged sleep profiles (right). nocte⁺ control flies are shown in blue and nocte¹ mutants that lack aristae (nocte¹ AL) are shown in red. The number of flies is shown j. j, Summary of locomotor activity rhythms under a ramping 18 °C to 25 °C temperature cycle and under constant conditions after entrainment for nocte⁺ flies and nocte¹AL mutants. Data are mean ± s.e.m. k, l, Double-plotted actograms of a representative nocte⁺ fly (k) and nocte¹ AL fly (l), which were entrained under temperature ramping cycles for 6 days and released into constant temperature (25 °C). The temperature gradient is shown at the bottom of the actogram. m, Locomotor activity averaged over four days of a 12 h:12 h light:dark cycle (left) and averaged sleep profiles (right) for nocte⁺ flies (blue) and nocte¹ AL flies (red). The number of flies is shown in n. Lights are turned on at ZT0 and turned off at ZT12. n, Summary of locomotor activity rhythms under light:dark (LD) and subsequent constant dark:dark (DD) conditions for nocte⁺ flies and nocte¹ AL mutants. Data are mean ± s.e.m. o, p, Double-plotted actograms of a representative nocte⁺ fly (o) and nocte¹ AL fly (p), which were entrained to 12 h:12 h light:dark cycles for 6 days and released into constant darkness. In all activity and sleep plots, dark lines indicate mean and shaded regions indicate s.e.m. Black dashed lines in the plots represent temperature.