Extended Data Figure 5: Activity and sleep profiles of wild-type Canton S flies under different amplitudes of ramping temperature cycles.
From: Circadian clock neurons constantly monitor environmental temperature to set sleep timing
a–g, Canton S flies were entrained to ramping temperature cycles of differing amplitudes and ranges: 18 °C to 28 °C or 22 °C to 28 °C for a week, followed by release into constant temperature conditions (25 °C) for another week. a, b, Averaged population activity of flies over the last four days of the temperature cycles (left) and averaged sleep plots under the same conditions (right). Dark lines indicate mean and shaded regions indicate s.e.m. n values are shown in d. c, Quantification of the anticipation of cooling for flies entrained to ramping temperature cycles from 18 °C to 25 °C, 18 °C to 28 °C and 22 °C to 28 °C. Data represent mean ± s.e.m. n values are shown in d. d, Summary of free-running locomotor activity rhythms of Canton S flies under constant conditions after entrainment to ramping temperature cycles. Data represent population average ± s.e.m. e–g, Averaged population locomotor activity on the day in which the temperature was unpredictably decreased (e, f) or increased (g) (left) and averaged sleep profiles (right) (see Methods) (n = 32 (e), n = 61 (f), n = 63 (g)). Blue bars indicate unanticipated cooling, red bars indicate unanticipated heating. Dark lines indicate mean and shaded regions indicate s.e.m. Black dashed lines in the plots represent temperature. n = number of flies.
