Abstract
Peptide-binding ligands would be useful for directing reagents to particular epitopes in a protein, the detection of peptide hormones, and many other applications. Here we show that peptides of modest size isolated from a library using a simple genetic assay can act as specific receptors for other peptides. The equilibrium dissociation constants of these peptide–peptide complexes are higher than those of typical monoclonal antibody–epitope complexes. Nonetheless, as shown here, these peptide-binding peptides can be used to detect or purify proteins containing the partner peptide.
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Acknowledgements
We thank Prof. James Hu (Texas A& M University) for many helpful discussions and for providing plasmids, phage, and bacterial strains. We also thank Liping Sun for providing purified S10-tagged Pho4 protein. This work was supported by grants from the Welch Foundation (I-1299) and the Texas Advanced Research Program (ARP 373) to T.K.
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Zhang, Z., Zhu, W. & Kodadek, T. Selection and application of peptide-binding peptides. Nat Biotechnol 18, 71–74 (2000). https://doi.org/10.1038/71951
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DOI: https://doi.org/10.1038/71951
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