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Transformation Systems for Generating Marker–Free Transgenic Plants

Bio/Technology volume 12, pages 263–267 (1994)Cite this article

Abstract

The ability to efficiently introduce foreign genes into plants is key to the success of the emerging plant biotechnology industry. Genetic transformation of crop plants is becoming increasingly routine both in terms of the number of plant species which can be transformed and the frequency of transformation, resulting in a number of transgenk products which are ready or close to market introduction1. The imminent commercialization of transgenk plants has generated debate about the desirability of the transgenk products containing selectable marker genes, or in fact any other ancillary DNA sequences not directly contributing to the final product2–6. In this review, we discuss these issues and examine transformation systems recently developed to selectively eliminate particular transgene sequences from the final transgenic plant.

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Authors and Affiliations

  1. Department of Vegetable Crops, University of California, Davis, CA, 95616

    John I. Yoder

  2. PBI Cambridge, Maris Lane, Trumpington, Cambridge, CB2 2LQ, UK

    Andrew P. Goldsbrough

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  1. John I. Yoder
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  2. Andrew P. Goldsbrough
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Yoder, J., Goldsbrough, A. Transformation Systems for Generating Marker–Free Transgenic Plants. Nat Biotechnol 12, 263–267 (1994). https://doi.org/10.1038/nbt0394-263

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