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A Chromosome Integration System for Stable Gene Transfer into Thermus flavus

Bio/Technology volume 13, pages 271–275 (1995) Cite this article

Abstract

We have developed a chromosomal integration system for gene transfer into the extreme thermophile Thermus flavus. The system relies on integration at the site of leuB (3-isopropylmalate dehydrogenase) which was cloned from T. flavus. The leuB gene was insertionally inactivated in vitro with a thermostable kanamycin-resistance gene and transformed in single-copy into the chromosome of T. flavus on a plasmid vector. Gene replacement strains required leucine for growth, were stably kanamycin-resistant and could grow hi the presence of kanamycin at temperatures up to 55°C.

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Author notes

  1. J. Mark Weber

    Present address: FermaLogic, Inc., 2201 W. Campbell Park Drive, Chicago, IL, 60612

  2. David C. Demirjian: Corresponding author.

Authors and Affiliations

  1. ThermoGen, Inc., 2201 W. Campbell Park Drive, Chicago, IL, 60612

    Scot P. Johnson, Veronika Vonstein & David C. Demirjian

  2. Department of Molecular Genetics and Cell Biology, University of Chicago, 920 E. 58th Street, Chicago, IL, 60637

    Malcolm J. Casadaban

Authors
  1. J. Mark Weber
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  2. Scot P. Johnson
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  3. Veronika Vonstein
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  4. Malcolm J. Casadaban
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  5. David C. Demirjian
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Weber, J., Johnson, S., Vonstein, V. et al. A Chromosome Integration System for Stable Gene Transfer into Thermus flavus. Nat Biotechnol 13, 271–275 (1995). https://doi.org/10.1038/nbt0395-271

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