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Regeneration of Transgenic Rose (Rosa hybrida) Plants from Embryogenic Tissue

Bio/Technology volume 12, pages 609–613 (1994)Cite this article

Abstract

Friable embryogenic tissues (FET) were recovered from filament cultures of rose, Rosa hybrida cv. Royalty, and cocultured with Agrobacterium tumefaciens or A. rhizogenes harboring a vector containing neomycin phosphotransferase II (NPTII) and β-glucuronidase (GUS) or firefly luciferase (LUC) genes. Putative transformed colonies were selected on kanamycin. Fifty–60 transformed embryogenic callus lines were reproducibly obtained from each gram of FET inoculated with Agrobacterium. Transformed embryogenic calli were transferred to maturation media to form somatic embryos, which subsequently produced flowering plants. The transgenic nature of the plants was confirmed by enzyme assays, polymerase chain reaction, and Southern hybridization. Over 100 transgenic plants have been established in soil and flowered in the greenhouse. This procedure facilitates introduction of desirable genes, especially those controlling flower color, into commercial cultivars of rose.

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Author notes

  1. Ebrahim Firoozabady: Corresponding author.

Authors and Affiliations

  1. DNA Plant Technology Corporation, 6701 San Pablo Ave., Oakland, California, 94608

    Ebrahim Firoozabady, York Moy, Neal Courtney-Gutterson & Karol Robinson

Authors
  1. Ebrahim Firoozabady
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  2. York Moy
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  3. Neal Courtney-Gutterson
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  4. Karol Robinson
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Firoozabady, E., Moy, Y., Courtney-Gutterson, N. et al. Regeneration of Transgenic Rose (Rosa hybrida) Plants from Embryogenic Tissue. Nat Biotechnol 12, 609–613 (1994). https://doi.org/10.1038/nbt0694-609

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