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Phage diabody repertoires for selection of large numbers of bispecific antibody fragments

Nature Biotechnology volume 14, pages 1149–1154 (1996)Cite this article

Abstract

Methods for the generation of large numbers of different bispecific antibodies are presented. Cloning strategies are detailed to create repertoires of bispecific diabody molecules with variability on one or both of the antigen binding sites. This diabody format, when combined with the power of phage display technology, allows the generation and analysis of thousands of different bispecific molecules. Selection for binding presumably also selects for more stable diabodies. Phage diabody libraries enable screening or selection of the best combination bispecific molecule with regards to affinity of binding, epitope recognition and pairing before manufacture of the best candidate.

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Author notes

  1. Hennie R. Hoogenboom

    Present address: CESAME at Dept. of Pathology, University Hospital Maastricht, Postbus5800, 6202 AZ, Maastricht, The Netherlands

  2. Gerald Walter

    Present address: Max Planck Institut fur Molekulare Genetik, Ihnestr. 73, D-14195, Berlin-Dahlem, Germany

  3. Alexander R. Duncan: e-mail: 100633.406@compuserve.com.

Authors and Affiliations

  1. Cambridge Antibody Technology Ltd., The Science Park, Melbourn, Cambridgeshire, SG8 6JJ, UK

    Brian T. McGuinness & Kevin FitzGerald

  2. Boehringer Mannheim Corporation, Research and Development, 9115 Hague Road, P.O. Box 50457, Indianapolis, IN, 46250–0457, USA

    Paula Schuler & Walt Mahoney

Authors
  1. Brian T. McGuinness
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  2. Gerald Walter
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  3. Kevin FitzGerald
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  4. Paula Schuler
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  5. Walt Mahoney
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  6. Alexander R. Duncan
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  7. Hennie R. Hoogenboom
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McGuinness, B., Walter, G., FitzGerald, K. et al. Phage diabody repertoires for selection of large numbers of bispecific antibody fragments. Nat Biotechnol 14, 1149–1154 (1996). https://doi.org/10.1038/nbt0996-1149

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