Supplementary Figure 3: TH2 differentiation of Thpokpd cells.
(a) ELISA quantification of IL-4 secretion by Thpokpd and Thpok+/+ cells activated under nonpolarizing conditions as in Fig. 3e. Each symbol represents a separate culture. Data are from three distinct sorting experiments, each with a distinct mouse from each genotype. The average IL-4 concentrations were 0.17 ng/ml for Thpok+/+ cells and <0.01 ng/ml for Thpokpd cells (*P < 0.005 by paired t-test). (b) Scatter plots show the percentage of IL-4 or IFNγ producers among Thpokpd or Thpok+/+ cells cultured under non polarizing conditions as in Fig. 3e. Each symbol represents a culture derived from a single mouse. Statistical significance was determined by t-test (*P < 0.005; **P < 10–4).
