Supplementary Figure 4: Functional relationships between ThPOK and Eomes and Cbfβ.

(a) Scatter plots show the percentage of IL 4- or IFNγ-producing cells among Thpok^+/+, Thpok^pd, Thpok^pd Cbfb^pd or Thpok^pd Eomes^pd cells cultured under nonpolarizing conditions as in Fig. 3e. Each symbol represents a culture derived from a single mouse. Statistical significance was determined by t-test (*P <0.05; **P < 0.025; ***P < 0.01) on pooled data from three independent experiments. Contours plots (b) show CD4 versus CD8 expression on total (left) or TCRβ^hiCD24^lo (center) thymocytes, or on TCRβ⁺ spleen cells (right) from 5 week old Thpok^+/+Eomes^+/+, Thpok^pd Eomes^+/+ or Thpok^pdEomes^pd mice. Representative of three or four mice for each genotype from two independent experiments. (c) Graphs show the absolute numbers of CD4⁺CD8^– and CD8⁺ T cells in the spleens from mice shown in b. There was no significant difference in the size of either subset among the three genotypes.