Supplementary Figure 2: T_H17 differentiation of Thpok^pd cells.

(a,b) Graphs show cumulative results for colon length (a) and mouse weight (b) from two independent C. rodentium infection experiments. Mean ± s.e.m. is shown. Each symbol represents a mouse. No significance by t-test. (c) ELISA quantification of IL-17 secretion by Thpok^pd and Thpok^+/+ cells activated under non-polarizing (T_HN), T_H1 or T_H17 conditions. Each symbol represents a separate culture. Data are from three mice of each genotype analyzed separately. Bars indicate mean ± s.e.m. No significance by t-test between Thpok^+/+ and Thpok^pd. (d) Histograms show GzmB expression in T effector cells obtained after 5-day cultures in T_H17 conditions of sorted CD4⁺CD44^lo spleen T cells from Thpok^+/+ (solid grey) or Thpok^pd (plain line) mice. The dashed line histogram shows GzmB expression on wild-type CD8⁺ effector cells cultured under T_HN conditions. Representative of three independent experiments.