Supplementary Figure 3: Overexpression of SULT2B1 inhibits TCR signaling. | Nature Immunology

Supplementary Figure 3: Overexpression of SULT2B1 inhibits TCR signaling.

From: Inhibition of T cell receptor signaling by cholesterol sulfate, a naturally occurring derivative of membrane cholesterol

Supplementary Figure 3

5C.C7 T cell blasts were transduced with SULT2B1 or control adenovirus with or without supplementation of the SULT2B1 substrate, 3'-phosphoadenosine-5'-phosphosulfate (PAPS). (a) Expression of SULT2B1 mRNA was determined by real-time PCR with the gene-specific TaqMan assay (Life technologies). (b) T cells were stimulated with the anti-CD3ɛ antibody 145-2C11 and TCR-induced phosphorylation of S6 protein was measured by phospho-flow cytometry. (c) T cells were stimulated with MCC-pulsed CH27 APC cells, and secreted IL-2 was determined by ELISA and presented in arbitrary units (AU). Data are from one experiment representative of three independent experiments. Unpaired t tests were performed resulting in the p values shown (n=5, mean with SEM).

Back to article page