Supplementary Figure 5: TCR signaling in DP thymocytes is more sensitive to CS than in SP thymocytes. | Nature Immunology

Supplementary Figure 5: TCR signaling in DP thymocytes is more sensitive to CS than in SP thymocytes.

From: Inhibition of T cell receptor signaling by cholesterol sulfate, a naturally occurring derivative of membrane cholesterol

Supplementary Figure 5

CD4+ SP (a), CD8+ SP (b) and DP thymocytes (c) treated with DMSO or CS at indicated concentrations. Pre-treated thymocytes were fluorescently labeled and mixed at a 1:1 ratio. Labeled cells were loaded with Indo-1 calcium-sensitive dye, and stimulated by adding anti-CD3ɛ 145-2C11 and anti-hamster IgG crosslinking antibodies. Indo-1 ratio indicating calcium flux was assessed by flow cytometry. Representative data of three experiments are shown. Black arrows indicate the time points of adding anti-CD3ɛ and crosslinking antibodies. Dashed green arrows highlight the differences of calcium flux between DMSO-treated (red) and CS-treated (blue) thymocytes.

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