Supplementary Figure 6: CS does not induce apoptosis in DP thymocytes of OT-1 Rag2−/− β2m-null mice. | Nature Immunology

Supplementary Figure 6: CS does not induce apoptosis in DP thymocytes of OT-1 Rag2−/− β2m-null mice.

From: Inhibition of T cell receptor signaling by cholesterol sulfate, a naturally occurring derivative of membrane cholesterol

Supplementary Figure 6

β2m-null mice were subjected to lethal dose radiation, then reconstituted with OT-I TCR transgenic Rag2−/− bone marrow. Six weeks after bone marrow transfer, DMSO control (left) or cholesterol sulfate (right) was injected intrathymically into the mice. After 2 days, Annexin V staining of DP thymocytes was analyzed by flow cytometry, as in Figure 5.

Statistical analysis for the percentage of Annexin V positive populations in DP thymocytes. Data are from one experiment representative of three independent experiments. Unpaired t tests were performed resulting in the p value shown on the right (mean with SEM, n=5).

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