Supplementary Figure 5: Kinetics of genomic occupancy by STAT1 in IFN-γ-stimulated BMDMs.
From: Opposing macrophage polarization programs show extensive epigenomic and transcriptional cross-talk
(a) BMDMs were stimulated with IFN-γ for 2 or 4 hours and STAT1 genomic occupancy measured by ChIP-Seq. Average enrichment plots and heatmaps were centered on the summit of STAT1 peaks. (b) A representative snapshot shows STAT1 recruitment at two IFN-γ-inducible loci (Rsad2 and Cmpk2). (c) Western blot analysis of phospho-STAT1 levels in macrophages stimulated with IFN-γ (+/- IL-4) for an extended kinetics. Phospho-STAT1 levels drop at 4 hours of IFN-γ stimulation.
