Supplementary Figure 6: Genomic occupancy of IRF1 in BMDMs stimulated with IFN-γ in the presence or absence of IL-4.
From: Opposing macrophage polarization programs show extensive epigenomic and transcriptional cross-talk
(a) Scatter plot showing IRF1 genomic occupancy after IFN-γ stimulation in the presence or absence of IL-4 co-stimulation. The Y-axis reports the -log10 of the FDR obtained by SICER when comparing IRF1 binding in BMDMs stimulated with IFN-g (4h) with IRF1 in unstimulated cells. The X-axis shows the log2 of the fold change in IRF1 measured in stimulated vs. costimulated cells. (b) Box plots showing the quantification of IRF1 ChIP-seq peaks in BMDMs stimulated with IFN-γ +/- IL-4 (left) and histone acetylation changes at the corresponding genomic regions (right). (c) Left: IRF1 peaks in proximity (+/- 50 kb) of IFN-γ inducible genes that are either sensitive or resistant to IL-4 co-stimulation. Right: box-plots showing the quantification of IRF1 ChIP-seq reads associated with IFN-γ-inducible genes that are sensitive or resistant to IL-4 co-stimulation.
