Supplementary Figure 1: General properties of IL-4-mediated inhibition of the transcriptional response to IFN-γ. | Nature Immunology

Supplementary Figure 1: General properties of IL-4-mediated inhibition of the transcriptional response to IFN-γ.

From: Opposing macrophage polarization programs show extensive epigenomic and transcriptional cross-talk

Supplementary Figure 1

(a) Dose-response analysis of IFN-γ- or IL-4-induced gene expression (4h stimulation). (b) Principal Component Analysis (PCA) and Pearson’s correlations for triplicate RNA-seq experiments. (c) Flow cytometry analysis by intracellular staining of BMDMs stimulated with vehicle (UT), IFN-γ, or IFN-γ+IL-4 for 8 hours. Percentages of CD11B+ NOS2+ cells are shown in boxes. (d) Dose-response analysis of the inhibitory effect of co-stimulation on genes induced by IFN-γ (used at 100 ng/ml) or IL-4 (10 ng/ml). (e) Repressive effect of IL-4 administered at the indicated time points after IFN-γ stimulation. RNA was collected after 4 hours of IFN-γ stimulation.

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