Supplementary Figure 7: Translation in effector CD8+ T cells generated in mice infected with either LCMV Arm or LCMV clone 13.
From: Translation is actively regulated during the differentiation of CD8+ effector T cells
(a) qRT-PCR quantification of Cd8a transcripts in fractions (horizontal axes) obtained by sucrose-gradient ultracentrifugation of lysates of Teff P14 CD8+ cells obtained from spleen of either LCMV Arm- or LCMV clone 13-infected mice (key) at day 8 post-infection. P14 cells were adoptively transferred before infection. This is a control of ribosomal-protein-encoding mRNAs in Fig. 8. (b) Amount of ribosomal-protein-encoding mRNA per cell in polysome fractions of D8 Teff cells obtained from LCMV-Arm- or LCMV-clone 13-infected mice (key); results are presented relative to those of LCMV Arm. Data (a) are representative of 5-6 independent experiments with samples pooled from 3-5 mice per each group. Data (b) were obtained from 5-6 independent experiments with samples pooled from 3-5 mice per each group. ****p<0.0001 (unpaired t-test). Each symbol in (b) represents an individual experiment.
