Abstract
We present a high-throughput method that enables efficient delivery of biomolecules into cells. The device consists of an array of 96 suspended electrode pairs, where small sample volumes are top-loaded, electroporated and bottom-ejected into 96-well plates. We demonstrate the use of this suspended-drop electroporation (SDE) device to effectively introduce fluorescent dextran, small interfering RNA (siRNA) or cDNA into primary neurons, differentiated neutrophils and other cell types with conventionally low transfection rates.
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Acknowledgements
We thank K. Merkle for machining the SDE device, D. Profitt for building the power supply, A. Hahn and P. Vitorino for careful reading of the manuscript, all the members of the Meyer lab for valuable discussions and advice. This work was financially supported by the Swiss Science Foundation and the US National Institute of Health.
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Guignet, E., Meyer, T. Suspended-drop electroporation for high-throughput delivery of biomolecules into cells. Nat Methods 5, 393–395 (2008). https://doi.org/10.1038/nmeth.1201
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DOI: https://doi.org/10.1038/nmeth.1201
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